MATEC Web Conf.
Volume 154, 2018The 2nd International Conference on Engineering and Technology for Sustainable Development (ICET4SD 2017)
|Number of page(s)||9|
|Published online||28 February 2018|
In vitro antimalarial activity of Calophyllum bicolor and hemozoin crystals observed by Transmission Electron Microscope (TEM)
Research Center for Chemistry, Indonesian Institute of Sciences (LIPI) PUSPIPTEK, Serpong, Indonesia
Objective : In continuation of our antimalarial candidate drug discovery program on Indonesia medicinal plants especially from stem bark of Calophyllum bicolor. Metode : We extracted of bioactive crude extract with hexane, acetone and methanol from stem bark of Calophyllum bicolor and evaluated their antimalarial activity by using parasite Plasmodium falciparum in vitro. Results: Methanol fraction showed most active and potent antimalarial activity dose dependent in in vitro experiments with IC50 = 0.52 ppb, and hexane fraction showed moderate antimalarial activity, but dose not dependent, while acetone fraction have not antimalarial activity. TEM (Trasmission Electron Microscopy) analysis confirmed a remarkable reduction of hemozoin in the presence of the bioactive fraction. Conclusion: The results suggest that the antimalarial activity of Calophyllum bicolor is due, methanol fraction showed the best in vitro antimalarial activity then another fraction.
Key words: Calophyllum bicolor / Plasmodium falciparum / in-vitro / TEM (Transmition Electron Mecroscope)
© The Authors, published by EDP Sciences, 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (http://creativecommons.org/licenses/by/4.0/).
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