MATEC Web Conf.
Volume 95, 20172016 the 3rd International Conference on Mechatronics and Mechanical Engineering (ICMME 2016)
|Number of page(s)||4|
|Section||Electronic Technology and Application|
|Published online||09 February 2017|
An Electrochemical DNA Sensor for p53 Tumor Suppressor Gene Detection
1 Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, China
2 NanChang Teachers College, JiangXi 330004, China
In this work, a sensitive electrochemical DNA sensor based on avidin modified electrode and DNA-functionalized Cds nanoparticle (DFNP) was developed. The DNA-Functionalized Cds nanoparticle contained two kinds of DNA, one was hairpin probe DNA with a biotin at the 3’’ terminal and a thiol at the 5’ terminal, the other is linearity signal DNA. Without hybridized with target DNA, the loop of hairpin impeded biotin linked with avidin on electrode. However, after target hybridization, hairpin was opened and biotin was recognized by avidin resulting in DNA-functionalized Cds nanoparticle was brought on electrode surface. Electrochemical signals of methylene blue (MB) bound to the signal DNA were measured by differential pulse voltammetry (DPV). Introduction By using this new method, we demonstrate that this prototype sensor has been able to detect as low as picomolar p53 tumor suppressor gene with excellent differentiation ability for even single mismatches.
© The Authors, published by EDP Sciences, 2017
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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