MATEC Web Conf.
Volume 67, 2016International Symposium on Materials Application and Engineering (SMAE 2016)
|Number of page(s)||6|
|Section||Chapter 1 Chemical Engineering|
|Published online||29 July 2016|
A New Electrochemical DNA Sensor for Sequence-Specific DNA Detection Based on DNA-functionalized CdS Nanoparticle
1 Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, China
2 NanChang Teachers College, JiangXi 330004, China
In this work, a sensitive electrochemical DNA sensor based on avidin modified electrode and DNA-functionalized CdS nanoparticle (DFCP) was developed. The DNA-Functionalized CdS nanoparticle contained two kinds of DNA, one was hairpin probe DNA with a biotin at the 3’ terminal and a thiol at the 5’ terminal, the other is linearity signal DNA. Without hybridized with target DNA, the loop of hairpin impeded biotin linked with avidin on electrode. However, after target hybridization, hairpin was opened and biotin was recognized by avidin resulting in DNA-functionalized CdS nanoparticle was brought on electrode surface. Electrochemical signals of methylene blue (MB) bound to the signal DNA were measured by differential pulse voltammetry (DPV). Introduction By using this new method, we demonstrate that this prototype sensor has been able to detect as low as picomolar DNA targets with excellent differentiation ability for even single mismatches.
Key words: DNA / sensor / MB / CdS nanoparticle
© The Authors, published by EDP Sciences, 2016
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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