MATEC Web of Conferences
Volume 32, 2015International Symposium of Optomechatronics Technology (ISOT 2015)
|Number of page(s)||4|
|Section||Progress in microscopy and imaging|
|Published online||02 December 2015|
Fluorescence microscope by using computational ghost imaging
1 Osaka university, Department of mechanical engineering, 560-0871 Osaka Japan
2 Tokushima university, Institute of technology and science, 770-8506 Tokushima Japan
3 JST/ERATO Minoshima Intelligent Optical Synthesizer Project, 770-8506 Japan
We propose a ﬂuorescence microscope by using the computational Ghost imaging (CGI) for observing a living cell for a long duration over an hour. There is a problem for observing a cell about light-induced bleaching fora ling-term observation.Toover come the problem, we focused on an advantageof sensitivityof the CGI as second order colleration for an imaging with weak intensity excitation light. Setting for the CGI, a DMD projector was installed at an eye-piece part of a microscope and ﬂuorescent light was detected using a bucket detectorofa photo-multiplier tube.Asaresults,wehaveshownthe imagingadvantageoftheCGI under weak light intensity, in addition, we have demonstrated to detect ﬂuorescence images of biological samples for one day.
© Owned by the authors, published by EDP Sciences, 2015
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