MATEC Web of Conferences
Volume 32, 2015International Symposium of Optomechatronics Technology (ISOT 2015)
|Number of page(s)||4|
|Section||Optomechatronics for Bio-Medical Applications|
|Published online||02 December 2015|
Parametric Optimization for High Speed FLIM Implementation
Department of Mechanical Engineering, KAIST, Daejeon, South Korea
a Corresponding author: firstname.lastname@example.org
FLIM (Fluorescence Lifetime Imaging Microscopy) has been considered an effective technique to investigate chemical properties of the specimens, especially of biological samples. Despite of this advantageous trait, researchers in this field have had difficulties applying FLIM to their systems because acquiring an image using FLIM consumes too much time. To increase the FLIM speed, many methodologies have been developed and applied to the system. One of the recent methodologies is an analogue mean delay based FLIM using a PMT and digitizer for image reconstruction. In this system, however, imaging time is largely dependent upon several parameters such as data transfer rate, sampling rate of an A/D converter, and signal width etc. In this paper, such parametric optimization method is introduced for faster acquisition of the image.
© Owned by the authors, published by EDP Sciences, 2015
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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