MATEC Web Conf.
Volume 238, 2018International Conference on Novel Functional Materials (ICNFM2018)
|Number of page(s)||5|
|Section||Biological Material and Process|
|Published online||26 November 2018|
Influence of the combination of last sense codon and stop codon on expression efficiency of green fluorescent protein gene in Escherichia coli when the expression vector pKK223-3
University of Science and Technology Liaoning, Anshan China, 114000
a Corresponding author: Haoxiaoliang1980@163.com
In this paper, the influence of the combination of last sense codon and stop codon on expression efficiency was studied. By our study, for the last sense codon CCG, the RFI of GFP(CCG) was 2.1 fold when the stop codon was UAA, but in comparison, the RFI was 1.1 fold when the stop codon was changed from UAA to UAG. For last sense codon TAG, the RFI of GFP(TAG) with the stop codon UAG was stronger than that with the stop codons UAA and UGA.
© The Authors, published by EDP Sciences, 2018
This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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