Effectivity comparison between aspirin, propolis, and bee pollen as an antiplatelet based on bleeding time taken on mice

¹ Department of Pharmacology and Theraupeutics Faculty of Medicine, Universitas Sumatera Utara, Jl. dr. Mansur No.5 Kampus USU, Medan, Indonesia
² Universitas Sumatera Utara Hospital, Jalan dr. T. Mansur No.66 Kampus USU, Medan, Indonesia.
³ Department of Public Health, Faculty of Medicine, Universitas Sumatera Utara, Jl. dr. Mansur No.5 Kampus USU Medan, Indonesia

^* Corresponding author: sake.martina@yahoo.com

Abstract

The use of standard antiplatelet therapy for cardiovascular disease patients has many side effects. This study aims to determine the effectiveness of Propolis and Bee Pollen, two bee products, as antiplatelet and in comparison with Aspirin. This is an experimental study with posttest only control group design. The samples were 32 mice, divided into 4 groups: placebo, administration of aspirin (10.4 mg/kg-bw/day), Propolis (65 mg/kg-bw/day), and Bee Pollen (68 mg/kg-bw/day). The study was conducted for 12 days and the suspensions were given orally. The bleeding time was then measured using tail-bleeding technique. The data collected was bleeding time in seconds. All samples completed the study. The mean bleeding time for placebo, Aspirin, Propolis and Bee Pollen groups were 106.1, 442.9, 310.1, and 203 in seconds respectively. ANOVA and Bonferroni tests showed a significant difference between Propolis and placebo groups (p=0.003), no difference between Propolis and Aspirin groups (p=0.100), no difference between Bee Pollen and placebo groups (p=0.442), and a significant difference between Bee Pollen and Aspirin groups (p=0.000). Administration of Propolis in mice showed prolongation of bleeding time while Bee Pollen did not prove to have antiplatelet effects.