MATEC Web Conf.
Volume 154, 2018The 2nd International Conference on Engineering and Technology for Sustainable Development (ICET4SD 2017)
|Number of page(s)||4|
|Section||Engineering and Technology|
|Published online||28 February 2018|
Pretreated of banana pseudo-stem as raw material for enzymatic hydrolysis and bioethanol production
Renewable Energy Research Centre, Department of Chemical Engineering, Universitas Muhammadiyah Surakarta, Indonesia
2 Department of Chemical Engineering, Universitas Muhammadiyah Surakarta, Indonesia
* Corresponding author: firstname.lastname@example.org
Development of alternative energy is needed to solve the energy problem, including bioethanol. Banana pseudo-stem is a lignocellulose material that can used to produce bioethanol. Banana pseudo-stem has 28.83% cellulose and 19.39% lignin. The amount of lignin will reduce by pretreatment process. Variations of pretreatment methods by autoclaving of banana-pseudo stem in a steam, 0.5N, 1N, 1.5N, 2N NaOH solutions for 90 minutes were employed. Then the preteated samples were further enzymatic hydrolysed for 24, 48, 72 hours. The fermentation method of simultaneous saccharification and fermentation (SSF) was applied using cellulase enzyme and yeast of Saccharomyces cerevisiae for 120 hours. The variation of the pretreatment process by increasing of NaOH concentration solutions led to decreased the lignin content while increased in cellulose content. The lowest lignin content was 11.44% and the highest cellulose was 51.66%. The highest sugar content was 29.8 g/L (at pretreatment 2N NaOH solution, 72 hours hydrolysis). The highest bioethanol amount (4.32 g/L) was produced from pretreated banana stem using 2N NaOH solution.
Key words: bioethanol / banana pseudo-stem / alkali pretreatment / enzymatic hydrolysis / SSF / Saccharomyces cerevisiae
© The Authors, published by EDP Sciences, 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (http://creativecommons.org/licenses/by/4.0/).
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