MATEC Web Conf.
Volume 100, 201713th Global Congress on Manufacturing and Management (GCMM 2016)
|Number of page(s)||4|
|Section||Part 4: Equipment manufacturing and New materials|
|Published online||08 March 2017|
Determination of Picogram Levels of Diacerein in a Pharmaceutical Formulation by Flow-Injection Chemiluminescence
School of Pharmacy, Xi’an Medical University, Xi’an 710021, China
A simple, sensitive and inexpensive method for determination of diacerein by flow-injection chemiluminescence was proposed, based on the quenching effect of diacerein on the luminol-protein (bovine serum albumin, BSA) reaction. It was found that the decrement of CL intensity was linearly proportional to the logarithm of diacerein concentration ranging from 5.0 to 7.0×103 pg·mL-1 (r = 0.9968), with the limit of detection (LOD) of 1.0 pg·mL-1 (3σ). The proposed procedure was successfully applied to the determination of diacerein in pharmaceutical formulation, human saliva, and serum samples without interference from its potential impurities, with the recoveries ranging from 96.4% to 104.0% and the relative standard deviations (RSDs) less than 4.0% (n=6).
Key words: diacerein / luminol / chemiluminescence / folw injection
© The Authors, published by EDP Sciences, 2017
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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