| Issue |
MATEC Web Conf.
Volume 95, 2017
2016 the 3rd International Conference on Mechatronics and Mechanical Engineering (ICMME 2016)
|
|
|---|---|---|
| Article Number | 02006 | |
| Number of page(s) | 4 | |
| Section | Material Structure and Measurement | |
| DOI | https://doi.org/10.1051/matecconf/20179502006 | |
| Published online | 09 February 2017 | |
A Surface Plasmon Resonance Histamine Sensor Based on an Electropolymerized Molecularly Imprinted Polymer (E-MIP)
Institute of Chemistry, University of the Philippines – Diliman, Quezon City, 1101 Philippines
In this paper, histamine sensing by surface plasmon resonance (SPR) spectroscopy using an electropolymerized molecularly imprinted polymer (E-MIP) of functionalized polyterthiophene and histamine is reported. The MIP histamine sensor showed good linear relationship (R2 = 0.995) between the SPR angle shift and the logarithm of histamine concentration, ranging from 15 – 500 μg/mL, with a limit of detection (LOD) of 2.0 μg/mL and a calibration sensitivity of 44.6 m° per log μg/mL histamine. Results also showed higher binding affinity of histamine for the MIP sensor than for the non-imprinted (NIP) sensor indicating excellent specificity. Moreover, the selectivity of the MIP histamine sensor against some of its structural analogues was demonstrated.
© The Authors, published by EDP Sciences, 2017
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