Facile Synthesis of Nitrogen-doped Carbon Quantum Dots for Bio-imaging

¹ Materials Science Division, Industrial Technology Development Institute, Department of Science and Technology, DOST Compound, Bicutan, Taguig City, Philippines
² chool of Graduate Studies, Mapua Institute of Technology, Muralla Street, Intramuros, Manila, Philippines
³ College of Engineering, University of the Philippines, Diliman, Quezon City, Philippines
⁴ Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathumthani, 12121 Thailand

^a Corresponding author: pandeyro@gmail.com

Abstract

Carbon quantum dots (CQD) with fascinating properties has gradually become a rising star as a new nanocarbon member due to its nonthreatening, abundant and inexpensive nature. This study reports on a facile preparation of fluorescent carbon quantum dots (CQD) from iota Carrageenan. CQD from iota Carrageenan was produced by hydrothermal method with a quantum yield (QY) of 16 to 20%. Doping the CQD with nitrogen by the addition of tetraethylene pentamine (TEPE) produced CQD with a QY of 77%. FTIR data confirmed the formation of hydroxyl, carboxylic and carbonyl functional groups as confirmed by the ToFSIMS data due to the presence of nitrogen bonds on the N-CQD produced with TEPE. The CQD and N-CQD produced are crystalline with graphitic structures because of the presence of sp2 graphitic d line spacing with the sizes ranging from 2 to 10nm. To examine the feasibility of using the CQD as nanoprobe in practical applications, labelling and detection of E.coli was performed. The E.coli fluoresced proving CQD as an effective probe in bio imaging application. This study has successfully demonstrated a facile approach of producing CQD with significant high quantum yields to fluorescent CQD for bio imaging applications.