| Issue |
MATEC Web of Conferences
Volume 22, 2015
International Conference on Engineering Technology and Application (ICETA 2015)
|
|
|---|---|---|
| Article Number | 05013 | |
| Number of page(s) | 7 | |
| Section | Chemical and Industrial Technology | |
| DOI | https://doi.org/10.1051/matecconf/20152205013 | |
| Published online | 09 July 2015 | |
Biotransformation and Production from Hansenula Anomala to Natural Ethyl Phenylacetate
School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, China
* Corresponding author: rong_sf@163.com
Ethyl phenylacetate can be widely applied in many industries, such as food, medicines, cosmetics and medicinal herbs. At the moment, the production of natural ethyl phenylacetate is very limited. However, the biotransformation production of natural ethyl phenylacetate has an very extensive application prospect. This paper is written by taking the phenylacetic acid tolerance and the esterifying enzyme activity as the two indexes for screening the HA14 strain of hansenula anomala mutagenic which is regarded as the microorganism of ethyl phenylacetate production through biotransformation. By optimizing the production condition of phenylacetic acid and the esterification condition of ethyl phenylacetate, the production of ethyl phenylacetate accomplished through biotransformation within 72 hours can reach 864mg/L which is 171% of that of the initial bacterial strain.
Key words: hansenula anomala / biotransformation / esterifying enzyme / ethyl phenylacetate
© Owned by the authors, published by EDP Sciences, 2015
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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