Synthesis of Enzyme-based Organic-Inorganic Hybrid Nanoflower Particles

¹ Department of Chemical and Energy, Curtin University Malaysia, CDT 250, 98009, Miri, Sarawak, Malaysia
² Department of Civil Engineering, Xi’an Jiaotong-Liverpool University, Suzhou, 215123, China
³ Department of Civil and Construction Engineering, Curtin University Malaysia, CDT 250, 98009, Miri, Sarawak, Malaysia
⁴ School of Chemical Engineering, Universiti Sains Malaysia, 14300 Nibong Tebal, Seberang Perai Selatan, Penang, Malaysia

^* Email: johnlsy@curtin.edu.my

Abstract

Enzyme-incorporated hybrid nanostructures are the immobilization of enzymes and inorganic components that exhibits promising characteristics in various industries. The immobilization of enzymes onto nanomaterial is naturally based to accommodate the enzymatic activity, stability, recyclability as well as their catalytic functions. The designing of these conjugates can improve the overall enzymatic performance by imparting their novel properties onto the system in comparison to conventional free enzymes which experience drawbacks in terms of deactivation or denaturing. A facile and ultrafast method is described in this paper to synthesize a novel enzyme-incorporated lipase/Cu₃(PO₄)₂ hybrid nanoflower (NF). The physical properties of the hybrid NF allow easier retrieval which indicates its higher reusability and recyclability value. The enzyme loading capacity was found to be 95.1% whereas, the catalytic performance of lipase/Cu₃(PO₄)₂ hybrid NF at the optimal conditions resulted in a specific enzyme activity of 1752 U/g corresponding to an increment of 90.5% to that of free lipase. This indicates that the well-designed lipase/Cu₃(PO₄)₂ hybrid NF to be highly efficient in industrial biocatalytic applications. Meanwhile, in future work, we aim to study its operational stability and reusability to enzymatically degrade biopolymers through hydrolysis process.