In-vitro biocompatibility testing of phycobiliproteins from Gracilaria changii

Department of Chemical Engineering, Adamson University, Manila, Philippines

Corresponding author: erison.roque@adamson.edu.ph

Abstract

The present study probes into the biocompatibility testing of Phycobiliproteins (PBPs) from Gracilaria changii, in-vitro. The cell survivability and cytotoxicity of PBPs to cultured Human Cervix Carcinoma (Hep-2C) cells were tested and observed using MTT (3-(4, 5- Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. For ten-fold dilutions, Phycobiliproteins (PBPs) promotes highest cell survivability at 10-4 dilution rate with 239.62% and highest cytotoxic effect, with 40.21%, at 10-1 dilution. The compositions of phycobiliproteins were determined using Ultraviolet (UV-Vis) spectrophotometer giving visible absorbance peaks at 498.5, 614 and 651 nm for the three PBPs composition: Phycoerythrin (PE), Phycocyanin (PC) and Allophycocyanin (APC), respectively. The effects of time duration in centrifugation process of Phycobiliproteins (PBPs) from Gracilaria changii in 3, 5 and 7 minutes, were proven significant on the quantity of the extracted PBPs. The highest average concentration of 20.37 µg/ml for APC, 13.31 µg/ml for PC and 18.57 µg/ml for PE were obtained at the 5 minute-extraction process.