MATEC Web Conf.
Volume 268, 2019The 25th Regional Symposium on Chemical Engineering (RSCE 2018)
|Number of page(s)||4|
|Section||Biochemical and Biomedical Engineering|
|Published online||20 February 2019|
Connected flow-through chromatography processes as continuous downstream processing of proteins
Bio-Process Engineering Laboratory, Graduate School of Medicine, Yamaguchi University Biomedical Engineering Center (YUBEC), Yamaguchi University, Tokiwadai, Ube, 755-8611, Japan
Corresponding author: firstname.lastname@example.org
Continuous manufacturing is expected to increase the productivity of protein drug production. However, it is not easy to build the continuous process especially for downstream processing as many unit operations (chromatography and membrane filtration) are involved. An operation method known as flow-through chromatography (FTC) is considered to be an efficient method for separating two components as the flow is continuous. In FTC, a target protein is eluted from the chromatography column without adsorption whereas contaminants are strongly bound. Since at least two different modes of chromatography are needed in order to remove contaminants, two FTC columns have to be connected in order to build the continuous process. This is not an easy task since the mobile phase properties (pH, salt, buffer ions) are different for the two columns. In this paper, we investigated how connected FTC columns can remove impurities efficiently from the cell culture broth containing monoclonal antibody. It was found that the sequence (activated carbon - anion exchange chromatography - cation exchange chromatography) is most efficient when the mobile phase pH and conductivity were properly chosen.
© The Authors, published by EDP Sciences, 2019
This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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