| Issue |
MATEC Web Conf.
Volume 197, 2018
The 3rd Annual Applied Science and Engineering Conference (AASEC 2018)
|
|
|---|---|---|
| Article Number | 13012 | |
| Number of page(s) | 4 | |
| Section | Environmental Engineering | |
| DOI | https://doi.org/10.1051/matecconf/201819713012 | |
| Published online | 12 September 2018 | |
Chlorpyrifos removal by Thiobacillus sp. and Clostridium sp. in liquid medium
Universitas Trisakti, Environmental Engineering Dapartement, Faculty of Landscape Architecture and Environmental Technology, Jakarta, Indonesia
* Corresponding author: astririnanti@trisakti.ac.id
The bioremediation research of chlorpyrifos has been done to remove the concentration of chlorphyrifos using the natural bacterial Thiobacillus sp. and Clostridium sp.. The efficiency of chlorpyrifos removal was determined by the temperature and contact time. The research was done by adding 100 ppm of chlorpyrifos into the Stone Mineral Salt solution (SMSs) under controlled condition, then each bacterial are added as much as 10% (v/v) with pH 7. To obtain optimum efficiency, this study was conducted with temperature variation (°C) 25, 30, 35, 40 and contact time (hours) 12, 24, 36, 48. Based on Gas Chromatography Mass Spektrofotometer (GC-MS) analysis, the efficiency of removal at temperature (°C) 25, 30, 35, 40 are 43%, 68%, 71% and 52% respectively, while the removal efficiency at contact time (hours) 12, 24, 36, 48 are 43%, 49%, 74%, and 36%. The result showed that 74% removal efficiency by Thiobacillus sp. and Clostridium sp. obtained at 35°C in 36 hours of contact time. Thus, the mixed culture of Thiobacillus sp. and Clostridium sp. are able to synergize for removing the chlorpyrifos at 100 ppm.
© The Authors, published by EDP Sciences, 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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