| Issue |
MATEC Web Conf.
Volume 175, 2018
2018 International Forum on Construction, Aviation and Environmental Engineering-Internet of Things (IFCAE-IOT 2018)
|
|
|---|---|---|
| Article Number | 01004 | |
| Number of page(s) | 4 | |
| Section | Construction Materials | |
| DOI | https://doi.org/10.1051/matecconf/201817501004 | |
| Published online | 02 July 2018 | |
Influence of culture conditions on extracellular polymeric substances production by the white rot fungi Phanerochaete chrysosporium
1
College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China
2
Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin 541004, China
* Corresponding author: aechoningjie_333@126.com
The extracellular polymeric substances of white rot fungi play an important role in the adsorption of heavy metals, but the influence of culture conditions on extracellular polymeric substances production is still unknown. In this paper, we researched on the influence of temperature, incubation time, the rotational speed and the inoculation volume on the yield of extracellular polymeric substances produced by Phanerochaete chrysosporium, a model strain of white rot fungi. The results show that the optimum culture conditions for Phanerochaete chrysosporium to produce extracellular polymeric substances was culturing at 40 °C, incubating for 5 d, rotating at 100 rpm, and inoculating 0.5 ml of spore suspension with concentration of 2.5×106 spores/ml. The highest yield of EPS was 234.65 mg/g when the fungi was cultured at 100 rpm, 40 °C and incubated for 5 days. This study can provide useful information for the follow-up experiments related to extracellular polymeric substances of white rot fungi
© The Authors, published by EDP Sciences 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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