CXCL-8 Regulates Head and Neck Carcinoma Progression through NOD Signalling Pathway

¹ Institute of Clinical Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
² Department of Otolaryngology-Head and Neck Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan
³ Division of Plastic Surgery & HBOT Center, Chi Mei Medical Center, Tainan, Taiwan
⁴ Department of Electrical Engineering, Southern Taiwan University of Science & Technology
⁵ Institute of Basic Medical Sciences, National Cheng Kung University, Tainan, Taiwan
⁶ Department of Cosmetic Science and Institute of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan
⁷ Department of Computer and Communication, Shu-Te University, Kaohsiung, Taiwan

^a Corresponding author: tinna_ling@mail.cnu.edu.tw

Abstract

Head and neck squamous cell carcinoma (HNSCC) ranks sixth among the most common cancers in the world. Interlukin-8 (CXCL-8), a major role in inflammatory response and tumor microenvironment, correlates with tumor progression, metastasis and invasion. We explored CXCL-8 promotes tumor progression in different differentiation HNSCC cells. This project would apply to development on biomarker and target in HNSCC as well as provide a basis of early diagnosis and treatment for clinical. CXCL-8, NOD1 (nucleotide-binding oligomerization domain-containing protein 1) and receptor-interacting protein kinase (RIPK2) levels were detected statistically higher in patient tissue with HNSCC than in non-cancerous matched tissue (NCMT) in the microarray and qRT-PCR study, whereas NOD2 was weakly expressed. Similar results were obtained for CXCL-8, NOD1, NOD2 and RIP2 from RT-PCR and western blotting. High CXCL-8, NOD1 and RIP2 expressions were found on HNSCC patient tissue than that of NCMT, whereas NOD2 was weakly expressed. The analytical results indicate that CXCL-8 is required in NOD 1-mediated signalling pathways in HNSCC.