MATEC Web Conf.
Volume 97, 2017Engineering Technology International Conference 2016 (ETIC 2016)
|Number of page(s)||10|
|Published online||01 February 2017|
Evaluation of Antioxidant Activity from Different Plant Parts of Senduduk Herb: Extraction Conditions Optimization of Selected Plant Part
Department of Chemical Engineering Technology, Faculty of Engineering Technology, Universiti Malaysia Perlis (UniMAP), Perlis, Malaysia
* Corresponding author: firstname.lastname@example.org
This work reports a study on evaluation of antioxidant properties from flower of Senduduk herb. Natural occurring antioxidant was mostly preferred due to their little or no toxicity compared to the synthetic antioxidants which posses carcinogenic effects. Extraction was done on selected plant parts of Sendududk herb including leaves, stem, flower and berry parts to evaluate their antioxidant potentiality. Flower part of Sendudk herb extracted using acetonic solvent promotes highest antioxidant activity which is 93.97 ± 1.38 % as compared to leaves (92.20 ± 0.20 %), stem (47.94 ± 1.42%) and berry (92.88 ± 0.63%) using the same extracting solvent. Thus, Senduduk flower was chosen to be continued with screening and optimization process. Single factor experiment using the one factor at a time (OFAT) method was done to study the effect of each extraction parameter that was solid to solvent ratio, temperature and solvent concentration. The extraction condition in each parametric study which results in highest antioxidant activity was used as the middle level of optimization process using Response Surface Methodology (RSM) coupled with Central Composite Design (CCD). The optimum condition was at 1:20 solid to solvent ratio, 64.61°C temperature and 80.24% acetone concentration which result in antioxidant activity of 96.53%. The verification of RSM showed that the model used to predict the antioxidant activity was valid and adequate with the experimental parameters.
© The Authors, published by EDP Sciences, 2017
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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