MATEC Web Conf.
Volume 85, 2016Chemistry and Chemical Technology in XXI Century (CCT 2016)
|Number of page(s)||9|
|Published online||17 November 2016|
Microbiological synthesis of methyl ethyl ketone
1 Tomsk Polytechnic University, Institute of natural resources, Tomsk, Russia
2 Tomsk State University, 634050 Tomsk, Russia
* Corresponding author: firstname.lastname@example.org
Optimal conditions for the microbiological synthesis of methyl ethyl ketone (MEK) on the mineral medium with butanol-1, butanol-2, 2-methylpropanol-2 by hydrocarbon-oxidizing bacteria Pseudomonas spp. were chosen. Optimal conditions for microbiological synthesis are liquid mineral Adkins’ medium containing 3% (vol.) of the substrate, stirring speed – 80-100 rev/min, temperature – 37° C, and synthesis time 24-48 hours. The specific growth rate and time of culture generation during the microbial synthesis were determined. It was found that methyl ethyl ketone is formed in the reaction mixture after 8, 48 and 72 hours when using 2-methylpropanol-2, butanol-2, butanol-1 as substrates, respectively. Maximum methyl ethyl ketone yield in the reaction mixture is observed after 12 hours in the case of using 2-methylpropanol-2 as a carbon source and accounts for 10%.
© The Authors, published by EDP Sciences, 2016
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