| Issue |
MATEC Web of Conferences
Volume 22, 2015
International Conference on Engineering Technology and Application (ICETA 2015)
|
|
|---|---|---|
| Article Number | 05020 | |
| Number of page(s) | 5 | |
| Section | Chemical and Industrial Technology | |
| DOI | https://doi.org/10.1051/matecconf/20152205020 | |
| Published online | 09 July 2015 | |
A Study and Application of Biocatalytic Synthesis of (S)-N-Boc-3-hydroxypiperidine
1 School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, China
2 College of Life and Environment Science, Shanghai Normal University, Shanghai, China
* Corresponding author: xuyi@sit.edu.cn
This paper first uses the environmental friendly whole cell of biocatalyst pichia pastoris SIT2014 to asymmetrically synthesized anti-tumor drug of chiral intermediate (S)-N-Boc-3-hydroxypiperidine.. Improve the final biocatalytic reduction yield to 85.4% based on the study of fermentation optimization and biocatalytic asymmetrical reduction system for pichia pastoris. The ee value of obtained reduction product (S)-N-Boc-3-hydroxypiperidine hits over 99%. The study of this article is a successful case where the biocatalyst is applied to the green synthesis of chiral intermediate of anti-tumor drug.
Key words: pichia pastoris / biocatalyst / (S)-N-Boc-3-hydroxypiperidine / asymmetrical reduction
© Owned by the authors, published by EDP Sciences, 2015
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.