| Issue |
MATEC Web Conf.
Volume 238, 2018
International Conference on Novel Functional Materials (ICNFM2018)
|
|
|---|---|---|
| Article Number | 04001 | |
| Number of page(s) | 5 | |
| Section | Biological Material and Process | |
| DOI | https://doi.org/10.1051/matecconf/201823804001 | |
| Published online | 26 November 2018 | |
Optimization of Fermentation Process Parameters for Ginsenoside Re Bioconversion by Plackett-Burman and Box-Benhnken Design
Department of Chemical and Engineering, University of Science and Technology of Liaoning, Anshan, Liaoning, 114051, China
a Xiuhong Wu: wxh93@163.com
In this work, Plackett-Burman (PB) and Box-Behnken (BB) experiment design were used to investigate the medium components of fermentation forth of ginsenoside Re bioconversion to ginsenoside Rh1. Firstly, nine variables including cultivation time, temperature, pH, reactant concentration, inoculum’s size, peptone, MgSO4*7H2O, K2HPO4 and NaNO3 were screened. The most important effective factors were estimated according to the statistical regression. Subsequently, the steepest ascent experiment was used to approach the optimum response range for each effective factor. Then Box-Benhnken Design (BBD) was finally used to analyze the response surface of the screened factors for further optimization. The optimized conditions for the influential factors were: ginsenoside Re 1.0 g/L, pH 4.5 and 7 days. The bioconversion of ginsenoside Re to Rh1 could reach up to 51.76 %.
© The Authors, published by EDP Sciences, 2018
This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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