Isolation and screening of resistant bacteria of heavy metal (Fe) at ship dismantling

¹ Institut Teknologi Sepuluh Nopember, Department of Environmental Engineering, Faculty of Civil, Environmental and Geo Engineering, Keputih, Sukolilo, 60111 Surabaya, Indonesia
² Institut Teknologi Sepuluh Nopember, Department of Ocean Engineering, Faculty of Marine Technology, Keputih, Sukolilo, 60111 Surabaya, Indonesia

^* Corresponding author: harminsulis@gmail.com

Abstract

The activity of ship dismantling is one of the sources of metal pollutant that polluted the environment. The aims of this study were analyze the iron concentration from the ship dismantling area, to isolate the bacteria from those area, and to determine the bacteria resistant on iron. Samplings was conducted in three point sampling at sea water and soil coast, respectively. Isolation of bacteria was carried out using pour plate methods. All isolated bacteria in seawater and soil samples were inoculated on nutrient agar medium (NA) containing ferrous metals (Fe²⁺) with various concentrations (0; 1,000; 2,000; 3,000; 4,000; 5,000; and 6,000 μg/mL). Based on the results, sea water and soil indicated that those area have contaminated with iron. The concentration of iron in seawater was 1.03, 1.01 and 1.00 μg/mL, respectively. Meanwhile, the concentration of iron in soil was 962.0, 966.05, 981.00 mg/kg, respectively. The result of qualitative and quantitative analysis showed that the isolates of AT, AL and CL coded bacteria have high resistance to the effect of iron. It indicated with clear zone of 6.00-7.00 mm at 6,000 μg/mL. In conclusion, both of bacteria are potential to be used for bioremediation of the ferrous metal (Fe²⁺) in further investigation.