Increased Yield of Biotransformation of Androsta-1, 4-Dien-3, 17-Dione from Β-Sitosterol by Using Sulfobutyl Ether-Β-Cyclodextrin Complexation Technique
School of Perfume and Aroma Technology, Shanghai Institute of Technology, 100 Haiquan Road, Fengxian District, Shanghai 201418, P. R. China
* Corresponding author: Shaofeng Rong and Qianqian Li
Substrate solubility in steroid biotransformation is critical for enhancing the biotransformation of hydrophobic compounds. In this study, the sulfobutyl ether-β-cyclodextrin (SBE-β-CD) complexation technique was used for the biotransformation of β-sitosterol to androsta-1, 4-diene-3, 17-dione with Mycobacterium ATCC25795. The production yield was increased by 26.72%, and the biotransformation course was shortened by 24h using β-sitosterol/SBE-β-CD inclusion complexes as substrates (1.0 g/L). Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that an inclusion complex was formed between SBE-β-CD and β-sitosterol. The complex significantly increased the solubility of β-sitosterol and improved the biotransformation efficiency of the substrate.
Key words: β-sitosterol / biotransformation / Mycobacterium / androsta-1 / 4-dien-3 / 17-dione
© The Authors, published by EDP Sciences, 2016
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