Fluorescence microscope by using computational ghost imaging
1 Osaka university, Department of mechanical engineering, 560-0871 Osaka Japan
2 Tokushima university, Institute of technology and science, 770-8506 Tokushima Japan
3 JST/ERATO Minoshima Intelligent Optical Synthesizer Project, 770-8506 Japan
We propose a ﬂuorescence microscope by using the computational Ghost imaging (CGI) for observing a living cell for a long duration over an hour. There is a problem for observing a cell about light-induced bleaching fora ling-term observation.Toover come the problem, we focused on an advantageof sensitivityof the CGI as second order colleration for an imaging with weak intensity excitation light. Setting for the CGI, a DMD projector was installed at an eye-piece part of a microscope and ﬂuorescent light was detected using a bucket detectorofa photo-multiplier tube.Asaresults,wehaveshownthe imagingadvantageoftheCGI under weak light intensity, in addition, we have demonstrated to detect ﬂuorescence images of biological samples for one day.
© Owned by the authors, published by EDP Sciences, 2015
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.