A Study and Application of Biocatalytic Synthesis of (S)-N-Boc-3-hydroxypiperidine
1 School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, China
2 College of Life and Environment Science, Shanghai Normal University, Shanghai, China
* Corresponding author: firstname.lastname@example.org
This paper first uses the environmental friendly whole cell of biocatalyst pichia pastoris SIT2014 to asymmetrically synthesized anti-tumor drug of chiral intermediate (S)-N-Boc-3-hydroxypiperidine.. Improve the final biocatalytic reduction yield to 85.4% based on the study of fermentation optimization and biocatalytic asymmetrical reduction system for pichia pastoris. The ee value of obtained reduction product (S)-N-Boc-3-hydroxypiperidine hits over 99%. The study of this article is a successful case where the biocatalyst is applied to the green synthesis of chiral intermediate of anti-tumor drug.
Key words: pichia pastoris / biocatalyst / (S)-N-Boc-3-hydroxypiperidine / asymmetrical reduction
© Owned by the authors, published by EDP Sciences, 2015
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